Metabolic fate of intravenously administered N-acetylneuraminic acid-6-14C in newborn piglets  

Oleh:

Wang, Bing ; Downing, Jeff A ; Petocz, Peter ; Brand-Miller, Jennie ; Bryden, Wayne L

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Asia Pacific Journal of Clinical Nutrition (keterangan: ada di Proquest) vol. 16 no. 01 (2007), page 110.
Topik: N-acetylneuraminic acid 6 14C; intravenous administration; brain; metabolic fate; newborn piglets

Ketersediaan

Perpustakaan FK Nomor Panggil: A27.K.2007.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: Sialic acid (N-acetylneuraminic acid), a component of gangliosides and sialylglycoproteins, may be a conditional nutrient in early life because endogenous synthesis is limited. The aim of this study was to investigate the metabolic fate of intravenously administrated N-acetylneuraminic acid 614C (sialic acid) in piglets. Method: Three-day-old male domestic piglets (Sus scrofa) were injected via the jugular vein with 5 µCi (11-12x106 cpm) of N-acetylneuraminic acid-614C (specific activity of 55 mCi/mmol). Blood samples were collected at regular intervals over the next 120 min. The organs were then removed and the urine collected for determination of residual radioactivity. Results: Within 2 min of injection, 80% of the activity was removed from the blood and by 120 min the remaining activity approached 8%. At 120 min, the brain contained significantly more radioactivity (cpm/g tissue) than the liver, pancreas, heart and spleen, but less than the kidneys. Within the brain, the percentage of total injected activity was highest in the cerebrum (0.175 ± 0.008) followed by the cerebellum (0.0295 ± 0.006, p = 0.00006) and the thalamus (0.029 ± 0.006, p = 0.00003). Conclusions: An exogenous source of sialic acid is capable of crossing the blood-brain barrier and being taken up into various tissues. The findings suggest that dietary sources of sialic acid may contribute to early brain development in newborn mammals.

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