Analysis of Acute Leukemias With MLL/ENL Fusion Transcripts: Identification of Two Novel Breakpoints in ENL  

Oleh:

Fu, Jen-Fen ; Liang, Der-Cherng ; Shih, Lee-Yung

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: American journal of Clinical Pathology vol. 127 no. 1 (Jan. 2007), page 24 - 30.
Topik: MLL/ENL; t(11; 19)(q23; p13.3); Acute myeloid leukemia; Acute lymphoblastic leukemia

Ketersediaan

Perpustakaan FK Nomor Panggil: A08.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

t(11;19)(q23;p13.3); is one of the common chromosomal translocations in acute leukemias involving MLL rearrangements. This translocation generates MLL/ENL fusion transcripts. In a study of acute leukemias, 148 patients were identified to have MLL rearrangements by Southern blot analysis. Reverse transcriptase-polymerase chain reaction (RT-PCR) assay, using primer sets covering the 2 previously described breakpoints at exons 2 and 7 of ENL detected 11 samples harboring MLL/ENL. complementary DNA panhandle PCR further identified 4 additional cases with novel breakpoints in ENL at exon 4 or 6. Sequencing analysis showed that all novel fusion transcripts were in-frame. The conventional cytogenetic analysis failed to detect t(11;19) in 6 of 13 cases. Of 15 patients with MLL/ENL, 7 had precursor B-cell acute lymphoblastic leukemia, 4 had T-cell acute lymphoblastic leukemia, and 4 had acute myeloid leukemia. The present study showed that PCR-based techniques are more sensitive than conventional karyotyping for detecting MLL/ENL fusions and an extra antisense primer at exon 6 of ENL should be included in RT-PCR assay to ensure complete detection of all MLL/ENL fusion transcripts.

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