Isolasi dan Karakterisasi Protein E Virus Dengue Isolat Surabaya sebagai Bahan Diagnostik  

Oleh:

Suwarno ; Rantam, Fedik Abdul ; Dachlan, Yoes Prijatna

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Penelitian Medika Eksakta vol. 03 no. 01 (Apr. 2002), page 49-56.
Topik: Dengue virus; Surabaya isolat; diagnostic materials
Fulltext: Suwarno.pdf (279.34KB)

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Perpustakaan PKPM Nomor Panggil: J101 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The aims of this resarch was to isolate and characterizate E protein of Dengue virus Surabaya isolate in order as diagnostic material. This research was divided into three steps isolation, characterization and analysis of protein E was performed by western blot technique using rabbit anti Den-3 polyclonal antibody. Protein E from gel electrophoresis was then isolated by SDS-PAGE. The result showed that molecular weight of E protein of Dengue virus Surabaya isolate was 52 kDa and it was antigenic protein which reacted specifically to rabbit anti Den-3 polyclonal antibody. Based on this result it was therefore can be conluded that by using SDS-PAGE technique E protein virus Dengue Surabaya isolate was antigenic protein with molecular weight 52 kDa.

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