Short Communication: Pretreatment Stress Enhances Embryogenic Callus Production in Anther Culture of Sturt's Desert Pea (Swainsona Formosa)  

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Zulkarnain

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 14 no. 1 (Mar. 2007), page 28-30.
Topik: Anther Culture; Embryogenic Callus; Stress Pretreatment; Swainsona Formosa
Fulltext: 99.pdf (175.02KB)

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Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.9 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The aims of the experiment were to investigate the effect of pretreatment stress on the proliferation of anther-derived embryogenic callus, and its subsequent embryo regeneration in Swainsonaformosa. Anthers were pretreated in starvation medium at cold (4 ‘C) temperature and cultured. Cultures were initially placed in darkness for four weeks, followed by 16-h photoperiod at 25 ± 1 “C. The stress pretreatment was found to enhance embryogenic callus formation on cultured anthers. Further, somatic embryos were successfully regenerated from embryogenic callus upon transferred onto regeneration medium supplemented with 1% sucrose and 4.63 mikroM kinetin, and later plantlets were obtained. Meanwhile, nonembryogenic callus continued to grow resulting in more callus mass without showing any embryoid initiation.

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