Characterization of Xylanase Streptomyces SPP SKK1-8  

Oleh:

Meryandini, Anja

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 13 no. 4 (Des. 2006), page 151-155.
Topik: Xilanase; Streptomyces SPP; Characterization; Zymogram and SDS-PAGE. Stability
Fulltext: 89.pdf (130.87KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.8 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 o~• Addition of 5 mM Cu2~ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 ‘C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 ‘C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan.

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