Tissue-Level Thresholds for Axonal Damage in An Experimental Model-of Central Nervous System White Matter Injury  

Oleh:

Meaney, David F. ; Bain, Allison C.

Jenis: Article from Bulletin/Magazine
Dalam koleksi: Journal of Biomechanical Engineering vol. 122 no. 6 (2000), page 615-622.
Topik: NERVOUS SYSTEMS; tissue - level; axonal damage; central nervous system; matter injury

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: JJ52.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

In vivo, tissue - level, mechanical thresholds for axonal injury were determined by comparing morphological injury and electrophysiological impairment to estimated tissue strain in an in vivo model of axonal injury. Axonal injury was produced by dynamically stretching the right optic nerve of an adult male guinea pig to one of seven levels of ocular displacement (N(level) = 10 ; N(total) = 70). Morphological injury was detected with neurofilament immunohistochemical staining (NF68, SMI32). Simultaneously, functional injury was determined by the magnitude of the latency shift of the N(35) peak of the visual evoked potentials (VEPs) recorded before and after stretch. A companion set of in situ experiments (N(level) = 5) was used to determine the empirical relationship between the applied ocular displacement and the magnitude of optic nerve stretch. Logistic regression analysis, combined with sensitivity and specificity measures and receiver operating characteristic (ROC) curves were used to predict strain thresholds for axonal injury. From this analysis, we determined three Lagrangian strain - based thresholds for morphological damage to white matter. The liberal threshold, intended to minimize the detection of false positives, was a strain of 0.34, and the conservative threshold strain that minimized the false negative rate was 0.14. The optimal threshold strain criterion that balanced the specificity and sensitivity measures was 0.21. Similar comparisons for electrophysiological impairment produced liberal, conservative, and optimal strain thresholds of 0.28, 0.13, and 0.18, respectively. With these threshold data, it is now possible to predict more accurately the conditions that cause axonal injury in human white matter.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali