MALDI-TOF-MS Analysis of Protein and DNA  

Oleh:

Bonk, Thomas ; Humeny, Andreas

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Neuroscientist vol. 7 no. 1 (Feb. 2001), page 6–12.
Topik: MALDI; Mass spectrometry; Neurochemistry; Genetic analysis; Protein analysis
Fulltext: 6TN71.pdf (99.82KB)

Isi artikel

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful and widespread analytical tool in all fields of life science. The wide mass range (1–300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectrophoresis, MALDI-TOF-MS is particularly suitable for the identification of protein spots via mass fingerprint or microsequencing. Furthermore, the method allows a detailed analysis of posttranslational protein modifications. Recently, the method was also successfully applied to DNA sequencing as well as screening for mutations. Thus, high-throughput genotyping of single nucleotide polymorphisms has the potential to become a routine method for both laboratory and clinical applications.

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