The Spread of Avian Reovirus 1091 in Cell Cultures is Inhibitied by Monoclonal Antibodies Agains tC Protein  

Oleh:

Astawa, Nyoman Mantik

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Mikrobiologi Indonesia vol. 11 no. 2 (Sep. 2006), page 72-76.
Topik: antibodies; avian reovirus; antibodies; protin

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: JJ139 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

A study on the in vitro inhibition of the cell to cell spread and avian reovirus 1091 induced cell fusion by monoclonal antibodies against tC protein of avian reovirus 1091 were produced by fusion of immortal mouse myeloma cells with lymphocytes derive from spleen of mice immunized with the virus. Five monoclonal antibodies (1A2, 4D3, 5A12, 5B11, and 5C6) against tC protein of avian reovirus 1091 were produced and used to inhibit the cell to cell spread and virus induced cell fusion in the infected cell cultures. All five monoclonal antibodies (1A2, 4D3, 5A12, 5B11, and 5C6) inhibited cell to cell spread of te virus in chick kidney cell cultures. Only four mnoclonal antibodies (1A2, 4D3, 5A12, and 5C6) inhibited the cell to cell spread od the virus in vero cells. Strong inhibition of cell to cell spread was exhibited by monoclonal antibodies 5A12 and 5C6, both chick kidney and vero cell cultures. Monoclonal antibodies 1A2, 4D3 and 5B11 only moderately inhibited cell to cell spread of the virus on chick kidney cells. Oly two monoclonal antibodies (5C6 and 5A12) inhibited the virus induced cell fusion both in primary chick kidney and vero check cultures. Monoclonal antibodies against tC protein inhibited the spread od the virus and virus induced cell fusio in cell cultures sugegsting that the protein plays an important role in avian reovirus infection. In addition the protein is likely to be a candidate for the preparation of a viable vaccine and antibodies against this protein is expected to limit the spread of the virus in the body

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