Fast Kinetic Analysis of Ligand-Gated Ion Channels  

Oleh:

Breitinger, Hans-Georg

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Neuroscientist vol. 7 no. 2 (Apr. 2001), page 95-103.
Topik: Ligand-gated ion channels; Kinetics; Func tion; Patch-clamp; Rapid ligand applica- tion
Fulltext: 95TN72.pdf (325.81KB)

Isi artikel

Ligand-gated ion channels mediate fast synaptic transmission in the central and peripheral nervous system and the neuromuscular junction. Their common prin ciple of func tion is the con version of a chemical signal—neurotransmitter binding—into an electrical signal, i.e., an ion influx into the postsynaptic cell. The transient nature of this signal requires experimental set ups that provide adequate tem poral resolution and the use of transient kinetic analysis rather than equilibrium methods for a correct description of receptor function. Although the highly specialized geometry of a synapse that allows very rapid delivery of neurotransmitter is difficult to mimic in an experimental system, a variety of techniques for rapid kinetic analysis are available, making it possible to determine at least some steps of receptor function with sufficient accuracy. This article provides an over view of strategies and methods of fast ligand application and kinetic analysis using whole-cell and single channel patch clamp. NEUROSCIENTIST 7(2):95–103, 2001

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