An Explosive Degrading Cytochrome P450 Activity and Its Targeted Application for The Phytoremediation of RDX  

Oleh:

Rylott, Elizabeth L. ; Jackson, Rosamond G. ; Edwards, James ; Womack, Grant L. ; Seth-Smith, Helena M.B. ; Rathbone, Deborah A. ; Strand, Stuart E. ; Bruce, Neil C.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 24 no. 2 (Feb. 2006), page 216-219.
Topik: gene; cytochrome; phytoremediation

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

The widespread presence in the environment of hexahydro 1,3,5 trinitro 1,3,5 triazine (RDX) one of the most widely used military explosives, has raised concern owing to its toxicity and recalcitrance to degradation. To investigate the potential of plants to remove RDX from contaminated soil and water, we engineered arabidopsis thaliana to experss a bacterial gene xplA encoding an RDX degrading cytochrome P450 (erf. 1). We demonstrate that the P450 domain of XplA is fused to a flavodoxin redoc partner and catalyzes the degradation of RDX in the absence of oxygen. Transgenic A. Thaliana expressing xplA was grown in RDX contaminated soil and found to be resistant to RDX phototoxicity, producing shoot and root biomasses greater than those of wild type plants. Our work suggests that expression of zplA in landscape plants may provide a suitable remediation strategy for sites contaminated by this class of explosives.

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