Derivation of Human Embryonic Stem Cells in Defined Conditions  

Oleh:

Thomson, James A. ; Ludwig, Tenneille E. ; Levenstein, Mark E. ; Jones, Jeffrey M. ; Berggren, W. Travis ; Mitchen, Erika R. ; Frane, Jennifer L. ; Crandall, Leann J. ; Daigh, Christine A. ; Conard, Kevin R. ; Piekarczyk, Marian S. ; Llanas, Rachel A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 24 no. 2 (Feb. 2006), page 185-187.
Topik: STEM CELL; stem cells

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) suppport feeder independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we repot feeder independent human ES cell culture that includes protein components solely derived from recombinant sources of purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

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