Gene Knockdown by Large Circular Antisense for High - Throughput Functional Genomics  

Oleh:

Yun-Han, Lee ; Ik-Jae Moon ; Bin, Hur ; Jeong-Hoh Park ; Kil-Hwan Han ; Seok-Yong, Uhm ; Yong-Joo, Kim ; Koo-Jeong Kang ; Jong-Wook Park ; Young-Bae, Seu ; Young-Ho, Kim ; Jong-Gu Park

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 5 (Mei 2005), page 591-600.
Topik: genomics; gene; circular antisense; genomics

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Single stranded genomic DNA of recombinant M13 phages was tested as an antisense molecule and examined to its usefulness in high-throughput functional genomics cDNA fragments of various genes (TNF-a , c-myc , c-myb, cdk2, and cdk4) were independently cloned into phagemid vectors. Using the life cycle of M13 bachteriphages, large circular (LC) molecules, antisense to their respective genes, were prepared from the culture supernatant of bacterial transformats. LC-antisense molecules exhibitid enhanced stability, target specificity and no need for target site searches. High troughput functional genomics was then attempted with an LC antisense library, which was generated by using a phagemid vector that incorporated a undirectional substracted cDNA library derived from liver cancer tissue. We identified 56 genes involved in the growth of these cells. These results indicate that an antisense sequence as a part of single stranded LC- genomic DNA of recombinant M13 phages exhibits effective antisense activity, and may have potential for high throughput functional genomics.

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