Stepwise Engineering to Produce High Yields of Very Long - Chain Polyunsaturated Fatty Acids in Plants  

Oleh:

Guohai, Wu ; Truska, Martin ; Datla, Nagamani ; Vrinten, Patricia ; Bauer, Joerg ; Zank, Thorsten ; Cirpus, Petra ; Heinz, Ernst ; Xiao, Qiu

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 8 (Agu. 2005), page 1013-1017.
Topik: ENGINEERING; engineering; polyunsatureated; fatty acids

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Very long chain polyunsaturated fatty acids (VLCPUFAs) such as arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are valuable commodities that provide important human health benefits. We report the transgenic production of significant amounts of AA and EPA in brassica juncea seeds via a stepwise metabolic engineering strategy. Using a series of transformations with increasing numbers of transgenes, we demonstrate the incremental production of VLCPUFAs, achieving AA levels of up to 25% and EPA levels of up to 15% of total seed fatty acids. Both fatty acids were almost exclusively found in triacylglycerols, with AA located preferentially at sn-2 and sn-3 positions and EPA distributed almost equally at all three positions. Moreover, we reconstituted the DHA biosynthetic pathway in plant seeds, demonstrating the pratical feasibility of large scale production of this important w-3 fatty acid in oilseed crops.

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