A Bacterial One - Hybrid System for Determining The DNA - Binding Specificity of Transcription Factors  

Oleh:

Xiangdong, Meng ; Brodsky, Michael H. ; Wolfe, Scot A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 8 (Agu. 2005), page 988-994.
Topik: dna; bacterial; one hybrid system; DNA; transcription factors

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.4 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The DNA binding specificities of transcription factors can be used to computationally predict this regulatory modules (CRMs) that regulate gene expression. However the absence of specificity data for the majority of transcription factors limits the widespread implementation of this approach. We have developed a bacterial one hybrid system that provides a simple and rapid method to determine the DNA binding specificity of a trancscription factor. Using this technology, we successfully determined the DNA binding specificity of seven previously characterized transcription factors and one novel trancription factor, the drosophila melanogaster factor odd skipped. Regulatory targets of odd skipped were successfully predicted using this information, demonstrating that the data produced by the bacterial one hybrid system are relevant to in vivo function.

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