Fruit - Specific RNAi - Mediated Suppression of DET1 Enhances Carotenoid and Flavonoid Content in Tomatoes  

Oleh:

Burgess, Diana ; Davuluri, Ganga Rao ; Tuinen, Ageeth van ; Fraser, Paul D. ; Manfredonia, Alessandro ; Newman, Robert ; Brumell, David A. ; King, Stephen R. ; Palys, Joe ; Uhlig, John ; Bramley, Peter M. ; Pennings, Henk M.J. ; Bowler, Chris

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 7 (Jul. 2005), page 890-895.
Topik: tomato verticulture; RNAi mediates; VET1; tomatoes

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Tomatoes are a principal dietary source of carotenoids and flvonoids, both of which are highly beneficial for human health. Overexpression of genes encoding biosynthetic enzymes or transcription factors have resulted in tomatoes with improved carotenoid or flavonoid content, but never with both. We attemtped to increase tomato fruit nutritional value by supressing an endogenous photomorphogenesis regulatory gene, DET1, using fruit specific promoters combined with RNA interference (RNAi) technology. Molecular analysis indicated that DET1 transcripts were indeed specifically degraded in transgenic fruits. Both carotenoid and flavonoid contents were increased significantly, whereas other parameters of fruit quality were largerly unchanged. These results demonstrate that manipulation of a plant regulatory gene can simultaneously influence the production of several phytonutrients generated from independent biosynthetic pathways, and provide a novel example of the use of organ specifi gene silencing to improve the nutritional value of plant derived products.

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