Multiplexed Detection of Pathogen DNA With DNA - Based Fluoresecence Nano Barcodes  

Oleh:

Younge, Li ; Yen, Thi Hong Cu ; Dan Luo

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 23 no. 7 (Jul. 2005), page 885-889.
Topik: pathogenicity; multiplexed detection; pathogen DNA; nanobarcodes

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Rapid, multiplexed, sensitive and specific molecular detection is of great demand in gene profiling, drug screening, clinical diagnostics and environmental analysis. One of the major challenger in multiplexed analysis is to identify each specific reaction with a distinct label or code. Two encoding strategies are currrently used : positional encoding, in which every potential reaction is preassigned a particular position on a solid phase support such as a DNA microarray and reaction encoding, where every possible reaction is uniquely tagged with a code that is most often optical or particle based. The micrometer size, polydispersity, complex fabrication process and nonbiocompatibility of current codes limit their usability. Here we demonstrate the synthesis of dendrimer like DNA based, fluorescence intensity coded nanobarcodes, which contain a built in code and a probe for molecular recognition. Their application to multiplexed detection of the DNA of several pathogens is first shown using fluorescence microscopy and dot blotting, and further demonstrated using flow cytometry that resulted in detection that was sensitive attomole and rapid.

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