High Resolution Genome Wide Mapping of Histone Modifications  

Oleh:

Tae-Young, Roh ; Wing, Chi Ngau ; Kairong Cui ; Landsman, David ; Keji Zhao

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 8 (Agu. 2004), page 1013-1016.
Topik: genome; high resolution genome; histone modifications

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The expression patterns of eukaryotic genomes are controlled by their chromatin structure, consisting of nucleosome subunits in which DNA of approxiamtely 146 bp is wrapped around a core of 8 histone molecules. Post translational histone modifications play an essential role in modifying chromatin structure. Here we apply a combination of SAGE and chromation immunoprecipitation (ChIP) protocols to determine the distribution of hyperacteylated histones H3 and H4 in the sacharomyces cerevisiae genome. We call this approach genome wide mapping technique (GMAT). Using GMAT we find that the highest acetylation levels are detected in the 5' end of a genes coding region, but not in the promoter. Furthermore, we show that the histone acetyltransferase, GCN5p, regulates H3 acetylation in the promoter and 5' end of the coding regions. These findings indicate that GMAT should find valuable applications in mapping target sites of chromatin modifying enzymes.

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