Post - Integration Stabilization of a Transposon Vector by Terminal Sequence Deletio In Drosophila Melanogaster  

Oleh:

Handler, Alfred M. ; Zimowska, Grazyna J. ; Horn, Carsten

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 9 (Sep. 2004), page 1150-1154.
Topik: STABILIZATION; post integration stabilization; transposon vector; terminal sequence deletion; drosophila melanogaster

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Germline transformation systems for nearly 20 insect species have been derived from transposable elements, allowing the development of transgenic insects for basic and applied studies. These system use a defective nonautonomous vector that results in stable vector integrations after the disappearance of transeintly provided transposase helper plasmid, which is essential to maintain true breeding lines and consistent transgene expression that would otherwise be lost after vector remobilization. The risk of remobilization by an unintended transposase source has so far not been a concern for laboratory studies, but the prospective use of millions of transgenic insects in biocontrol programs will likely ecological safety of field release programs. Here we describe an efficient method that deletes a terminal repeate sequence of a transposon vector after genomic integration. This procedure prevents transposase mediated remobilization of the other terminal sequence and associated genes, ensuring their genomic stability.

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