Temporal Analysis of Phosphotyrosine - Dependent Signaling Networks by Quantitative Proteomics  

Oleh:

Blagoev, Blagoy ; Shao-En, Ong ; Kratchmarova, Irina ; Mann, Matthias

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 9 (Sep. 2004), page 1139-1145.
Topik: networks; temporal analysis; phosphptyrosine; networks quantitative proteomics

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

To study the global dynamics of phosphotyrosine based signaling events in early growth factor stimulation, we developed a mass spectrometric method that converts temporal changes to differences in peptide isotopic abundance. The proteomes of three cell populations were metabolically encoded with different stale isotopic forms of arginine. Each population was stimulated by epidermal growth arginine. Each population was stimulated by epidermal growth factor for a different length of time, and tyrosine phosphorylated proteins and closesly associated binders were affinyity purified. Arginine containing peptidesoccured in three forms, which were quantified, we then combined two experiments to generate five point dynamic profiles. We identified 81 signaling proteins, including virtually all known epidermal growth factor receptor sustrates, 31 novel effectors and the time course of their activation upon epidermal growth factor stimulation. Global activation profiles provide an informative perspective on cell signaling and will be crucial to modeling signaling networks in a systems biology approach.

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