Clonal Identification of Multipotent Precursors from Adult Mouse Pancreas That Generate Neural and Pancreatic Lineages  

Oleh:

Seaberg, Raewyn M. ; Smukler, Simon R. ; Kieffer, Timothy J. ; Enikolopov, Grigori ; Asghar, Zeenat ; Wheeler, Michael B. ; Korbutt, Gregory ; Kooy, Derek van der

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 9 (Sep. 2004), page 1115-1124.
Topik: COLONIES; clonal; multipotent; mouse pancreas; neural; pancreatic lineages

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The clonal isolation of putative adult pancreatic precursors has been an exlusive goal of researchers seeking to develop cell replacement strategies for diabetes. We report the clonal identification of multipotent precursor cells from the adult mouse pancreas. The application of a serum free, colony forming assay to pancreatic cells enable the identification of precursors from pancreatic islet and ductal populations. these cells proliferate in vitro to form clonal colonies that coexpress neural and pancreatic precursor markers. Upon diferentiation individual clonal colonies produce distinct populations of neurons and glial cels, pancreatic endocrine b-, a- and d- cells, and pancreatic exocrine and stellate cells. Moreover the newly generated b-like cells demonstrate glucose dependent Ca (2+) responsiveness and insulin release. Pancreas colonies do not express markers of embryonic stem cels, nor genes suggestive of mesodermal or neural crest origins. These cells represent a previously unidentified adult intrinsic pancreatic precursor population and are a promising candidate for cell based therapeutic strategies.

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