Photoswitchable Cyan Fluorescent Protein for Protein Tracking  

Oleh:

Chudakov, Dmitriy M. ; Verkhusha, Vladislav V. ; Staroverov, Dmitry B. ; Souslova, Ekaterina A. ; Lukyanov, Sergey ; Lukyanov, Konstantin A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Nature Biotechnology: The Science and Business of Biotechnology vol. 22 no. 11 (Nov. 2004), page 1435-1439.
Topik: PROTEIN; photoswitchavle; cyan fluorescent protein; protein tracking

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: NN9.3 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

In recent years diverse photolabeling techniques using green fluorescent protein (GFP) like proteins have been reported including photoactivatable PA-GFP, photoactivatable protein Kaede the DsRed greening technique and kindiing fluorescent proteins. So far, only PA-GFP which is monomeric and gives 100 fold fluorescence contrast, could be applied for protein tracking. here we describe a dual color monomeric protein, photoswitchable cyan fluorescent protein (PS-CFP). PS-CFP is capable of efficient photoconversion from cyan to green, changing both its excitation and emission spectra in response to 405 nm light irradiation. Complete photoactivation of PS-CFP resultsd in a 1,500 fold increase in the green to cyan fluorescence ratio, making it the highest contrast monomeric photoactibatable fluorescent protein described to date. We used PS-CFP as a photoswitchable tag to study trafficking of human dopamine transporter in living cells. At moderate excitation intensities. PS-CFP can be used as a pH-stable cyan label for protein tagging and fluorescence resonance energy transfer application.

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