Isolasi dan Analisis Keragaman Genetik Escherichia Coli pada Makanan Jajanan Berdasarkan Sekuen Eric - PCR  

Oleh:

Lindawati ; Waturangi, Diana Elizabeth ; Soka, Susan Sundari

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI - atma jaya
Dalam koleksi: Atma nan Jaya vol. 21 no. 1 (Jan. 2006), page 24-39.
Topik: GENETIKA; Street Food; Serology Test; Sanitation
Fulltext: Diana E. Lindawati.pdf (175.08KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: AA48 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan PKPM Nomor Panggil: A66 Non-tandon: 2 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Escherichia coli is a commensal bacteria living in the human and animal digestitive tracts. Many studies have been conducted on E. coli isolated from human, animal, food, and water, while little is known about the presence and genetic diversity of E. coli obtained from street foods. If the street foods are not handles or processed properly, they may endanger the health of the children, because E. coli contamination has known to be an agent causing diarrheal disease. In this study, E. coli isolates from street foods of elementary school in jakarta especially north and south jakarta were isolated and were confirmed with the specific biochemistry and serology test. The serology test was done using polivalent O1 - O11 antisera, monovalent O111 and 026 antisera for E. coli. Genetic diversity analysis was done using enterobacterial repetitive intergenic consensus (ERIC) - PCR. thirteen E. coli isolates were found from meals and thirty-fourth from beverages. One isolate performed positive result in serology test using O111. This study showed that street foods from Jakarta elementary school are poor in sanitation and quality. While for genetic diversity analysis, E. coli isolates showed an extensive genetic diversity even for isolates from the same sample. The result indicated that ERIC-PCR analysis could be useful and effective tool for rapid study of genetic.

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