Daya Hidup Spermatozoa Epididimis Domba Garut yang Dikriopreservasi Menggunakan Modifikasi Pengencer Tris  

Oleh:

Rizal, Muhammad ; Herdis

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 12 no. 2 (Jun. 2005), page 61-66.
Topik: spermatozoa; Spermatozo; Epididimis; Kriopreservasi
Fulltext: 41.pdf (57.49KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.7 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Sperm collected from cauda epididymis is a source of male gametes. The purposes of this study was to evaluate an quality of frozen-thawed sperm of garut ram which was collected from cauda epididimys and cryopreserved with modified Tris extender, i. e.: Tris extender (control, KT), Tris extender + 60 mM lactose (LS), and Tris extender + 60 mM lactose = 0,05% glutathione (GL). Quality of collected sperm including concentration, motility, live sperm, abnormality, cytoplasmic droplet, intract acrosomal cap (IAC), and intact plasma membrane (IPM) were evaluated. Results showed that mean of sperm concentration, percentage of motility, live sperm abnormality, cytoplasmic droplet, IAC, and IPM of fresh epididymal sperm were 13,99.33 milion/ml, 70.83, 82.83, 10.83, 8.5, 85.83, and 81.33% respectively. Sperm quality after equilibration for LS and GL were significantly (P<0.05) higher than K.T. Mean percentages of post thawing sperm motility, live sperm, IAC, and IPM for GL (45, 54.5, 47.83 and 48.3%) were significantly (P<0,05) higher than LS (40, 49.17, 43.83,and 44.5%), and KT (35, 42.5, 39.17, 41.5%). Mean percentages of post thawing sperm motility, live sperm, IAC, and IPM for LS were significantly (P<0.05) higher than those of K. T. Hence, frozen-thawed epididymal sperm of garut ram after slaughter and cryopreserved with Tris extender + 60 mM lactose (LS) and Tris extender + 60 mM lactose + 0,05% glutathione (GL) possibly can be used for artificial insemination (AI) or in vitro embryo production program.

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