Detection of Hepatitis B Virus X Gene Mutation from Local Clinical Samples  

Oleh:

Artarini, Anita ; Jessica, Hanary Geby ; Kartikasari, Raden Rini ; Riani, Catur ; Retnoningrum, Debbie Soefie

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 10 no. 1 (Mar. 2016), page 9-14.
Topik: hepatitis B virus; Indonesia; X gene mutation
Fulltext: 374-1068-1-PB_Ros.pdf (350.42KB)

Isi artikel

Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and it infects hepatocytes, which is the most common cell in liver. HBV infection is distinguished into acute and chronic infection based on the duration of infection. Chronic infection of HBV exists more than six months and it can develop into liver cirrhosis and hepatocellular carcinoma (HCC). The development of chronic HBV infection is affected by viral particle load, genotype and subgenotype, as well as its association with HBV X protein (HBx). Several mutations of the HBx gene are known to be associated with HCC. The aim of this study is to determine the genotype and subgenotype based on HBx gene, and to detect its mutation from 44 local clinical samples. The HBx gene was amplified using nested PCR, which produced two fragments with size of 469 and 395 bp. The obtained HBx gene sequences were aligned with HBx gene sequences from database to determine the genotype, subgenotype, and amino acid substitution. The analysis showed that patients were infected mainly by HBV subgenotype B3, which are common in Indonesia. In this study, we found the presence of known HCC-related HBx mutant, i.e. V5L, A47T, I127T and K130M/V131I, as well as new HBx mutant, i.e. G22S and A85T. The presence of HBx T118N mutant was detected at the highest percentage and occurred from samples with high HBV DNA titer. Detection of HBx gene mutations might be necessary to predict the manifestation of liver disease, as well as development of HCC.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali