Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation  

Oleh:

Wibowo, Sara Gustia ; Helianti, Is ; Suryani, Ani ; Wahyuntari, Budiasih

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 10 no. 3 (Sep. 2016), page 112-117.
Topik: Bacillus halodurans CM1; production optimization; RSM; xylanase
Fulltext: 501-1253-1-PB_Ros.pdf (692.51KB)

Isi artikel

A two level factorial design was performed to optimize xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X ), 1 nitrogen source (X ) concentration, and pH (X ), corn cob and fish powder were used as carbon and nitrogen 2 3 source respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production. A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X ) and pH (X ). The optimum corn cobs concentration was 4.37% (w/v), 1 3 and the fish powder (with high P content) concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity observed was 522 U mL-1 after 24 h fermentation.

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