The Synergistic Effect of Doxorubicin and Ethanolic Extracts of Caesalpinia sappan L. Wood and Ficus septica Burm. f. Leaves on Viability, Cell Cycle Progression, and Apoptosis Induction of MCF7 Cells  

Oleh:

Haryanti, Sari ; Pramono, Suwijiyo ; Murwanti, Retno ; Meiyanto, Edy

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 21 no. 1 (2016), page 29–37.
Topik: Sappan wood; Ficus septica leaves; doxorubicin; MCF7; cell cycle; apoptosis
Fulltext: 26105-56417-2-PB_Ros.pdf (1.23MB)

Isi artikel

Caesalpinia sappan L. and Ficus septica Burm. f known as a potential plant with wide variety of medicinal properties, including anticancer. Present study was aimed to explore cytotoxic effect of sappan wood (ECS) and awarawar leaves (EFS), and its combination with doxorubicin (dox) on MCF7 cells focusing on cell cycle progression and apoptosis induction. The result of MTT assay showed that single treatment of ECS and dox performed cytotoxic effect with the IC50 value of 32 µg/mL and 6 µM respectively, while EFS performed low cytotoxic effect with the IC50 value of 282 µg/mL. The combination of ECS with EFS and doxorubicin showed synergistic cytotoxic effect. Flow cytometry analysis revealed that combination of ECS (16 µg/mL) with EFS (8 µg/mL) and doxorubicin (2 µM) induced apoptosis, and cell accumulation at subG1 and G2/M phases. Immunoblotting assay confirmed the apoptosis induction of this combination through increasing of cleavage of PARP1. Based on these results, the synergistic cytotoxic effect of this combination was through G2/M phase accumulation and apoptosis induction and potentially to be developed as cochemotherapeutic agent.

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