Differences of Lymphocyte Proliferation Index After Culture Filtrate Protein 10 (CFP-10) Stimulation in Patients with Active and Latent Tuberculosis and Healthy Individuals  

Oleh:

Utami, Binar R. ; Agustina T., Betty ; Ma’at, Suprapto

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 02 (Mar. 2017), page 184-190.
Topik: CFP-10; Lymphocytes Proliferation Index; Active Pulmonary TB; Latent TB; Healthy People
Fulltext: I01 v23 n2 p184 kelik2017.pdf (508.03KB)

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Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The incidence of tuberculosis in the world is increasing, due to the low effectiveness of the BCG vaccine for the prevention of TB infection. Currently, a DNA vaccine from M.tuberculosis proteins has been development that is a Culture filtrate Protein 10 (CFP-10) which can stimulate cellular immune response. The purpose of this study was to determine one of the abilities of CFP-10 antigen to stimulate an immune response in patients with active TB, latent TB and healthy people. This study was a Quasi-experimental study. Samples were Peripheral Blood Mononuclear Cell (PBMC) from 10 patients with active pulmonary tuberculosis, 10 latent TB at the Karang Tembok Pulmonary Hospital, Surabaya and 10 healthy people. Peripheral blood mononuclear cell cultures without stimulation (control), with mitogen (PHA) as a positive control and with CFP-10 antigen, incubated at 5% CO2 for 5 days. Lymphocyte proliferation test was conducted by adding MTT. Proliferation index measured by comparing the absorbant with antigen stimulation control and absorbant ? 560 nm. There was a significant difference in lymphocyte proliferation index post stimulation CFP-10 between active pulmonary tuberculosis patients and healthy individuals (p=0.019) and in patients with active TB, latent TB and healthy individuals (p=0.0356). CFP 10 antigen had the ability of inducing a protective immune response in patients with active TB new, latent TB and healthy people. Immunogenic antigens CFP-10 activities can be influenced by a person’s immune status and the ability to induce a protective immune response.

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