Comparison Results of Analytical Profile Index and Disc Diffusion Antimicrobial Susceptibility Test to Technical Dedicated Reasonable 300B Method  

Oleh:

Sugiartha, Eka ; Rejeki, Putri Sri ; Wardhani, Puspa ; Semedi, Bambang Pujo

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 23 no. 02 (Mar. 2017), page 132-138.
Topik: Comparison; API; Disc Diffusion; TDR-300B
Fulltext: I01 v23 n2 p131 kelik2017.pdf (402.88KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The bloodstream infection death rate is quite high, ranging from 20% to 50%. Causable pathogens could be demonstrated by blood cultures followed by antimicrobial susceptibility tests. The test could be performed in a manual, semiautomatic or fully automatic method. The manual method does not require large investment costs compared to automatic methods. This was an observational cross sectional design study. Manual identification method used API and antimicrobial susceptibility tests used disc diffusion method of Kirby Bauer. Both methods were compared to semiautomatic TDR-300B method. A fully automatic VITEK 2 method was used as the reference method for assessing the performance of manual and semiautomatic methods. Bacteria that caused bloodstream infections were mostly dominated by Gram-negative Escherichia coli and Klebsiella pneumonia. The accuracy of API identification method to the VITEK 2 was 87.87%, accuracy of TDR-300B identification method to VITEK 2 method was 90.9%. The accuracy results of manual Kirby Bauer disc diffusion antimicrobial susceptibility tests method compared to VITEK 2 method was 84.64%. Accuracy of TDR-300B antimicrobial susceptibility tests method to VITEK 2 was 82.5%. The accuracy of API identification method to TDR-300B was 84.84%.The accuracy of manual antimicrobial susceptibility test method to TDR-300B was 78.21%. The results of manual identification and antimicrobial sensitivity tests were not statistically significanly different with semiautomatic TDR-300B method. Manual identification and antimicrobial susceptibility test methods could be trusted, especially for financial limited region or small number of examination.

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