Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium  

Oleh:

Hidayat, Chusnul ; Darmasiwi, Sari ; Nurikasari, Maulina ; Cahyanto, Muhammad Nur

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 20 no. 2 (Dec. 2015), page 108-116.
Topik: Lipase; Aspergillus niger; jatropha cake; solid state fermentation
Fulltext: 24195-48078-1-SM.pdf (527.36KB)

Isi artikel

Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potential as a medium for lipase production. The objective of this research was to characterize lipase from Aspergillus niger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specificity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specificity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and vmax about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.

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