Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia  

Oleh:

Yohandini, Heni ; Julinar ; Muharni

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 22 no. 03 (Jul. 2015), page 143-148.
Topik: phylogenetic analysis; Tanjung Sakti Hot Spring; thermophile
Fulltext: 10776-31091-1-SM.pdf (1.93MB)

Isi artikel

A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80
Ce91
C and pH 7e8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank. Copyright © 2015 Institut Pertanian Bogor. Production and hosting by Elsevier B.V. This is an open

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