Cytotoxic Activities of Ethyl Acetate Fractions from Petroleum Ether Exctract and Methanol Extract of Pistiae Leaves  

Oleh:

Raharjo, Sentot Joko ; Ningsih, Rahayu Wahyu

Jenis: Article from Journal - ilmiah internasional - tidak terdaftar di DIKTI
Dalam koleksi: Majalah Obat Tradisional vol. 20 no. 03 (Sep. 2015), page 134-139.
Topik: BSLT; Ethyl Acetate Partition; Methanol Extract; Petroleum Ether Extract; Pisteae Folium; LC50
Fulltext: M01 v20 n3 p134 kelik2017.pdf (787.56KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: M01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

This study aims to explore the cytotoxic test (LC50) towards Artemia salina L. larvae of the partition ethyl acetate fractions from methanol extracts and petroleum ether (PE) extracts of Pistiae folium. The leaves of Pistiae folium was extracted using solvents soxhletation with methanol solvent and petroleum ether solvent. Both extracts successively partitioned with n-hexane and ethyl acetate, respectively. Identification of secondary metabolites was using phytochemical screening method and TLC (Thin Layer Chromatography). TLC analysis was using the eluent chloroform: methanol 3: 1 by citroboric acid spot analysis. The cytotoxic activity was using Brine Shrimp Lethality Test (BSLT) method. The result of partition from methanol extracts was 6.25% and from PE extracts was 0.69%. The phytochemical screening test showed that it contained flavonoids, saponins and steroids. The TLC analysis identified flavonoid compounds. The cytotoxic activity (LC50) of the ethyl acetate partition from methanol extracts and petroleum ether extracts of Pistiae folium were 79.9298mg/mL and 51.7608mg/mL, respectively. The result showed that the cytotoxic activity of ethyl acetate fractions partitined from methanol extract of Pistiae folium was higher than the PE extracts.

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