Isolation and Determination of Kembang Sepatu Flowers (Hibiscus rosa-sinensis L.) Ethanolic Extract Using Spectrodensitometry  

Oleh:

Pamungkas, Kurniati ; Murrukmihadi, Mimiek

Jenis: Article from Journal - ilmiah internasional - tidak terdaftar di DIKTI
Dalam koleksi: Majalah Obat Tradisional vol. 20 no. 02 (May 2015), page 112-118.
Topik: H. Rosa-Sinensis Flowers; Compound Isolation; Concentration Determination; Alkaloid; Spectrodensitometry
Fulltext: M01 v20 n2 p112 kelik2017.pdf (544.78KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: M01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

This research aims to isolate and determine relative concentration of alkaloid in Hibiscus rosa-sinensis L flowers. using spectrodensitometry. Ethanolic extract of H. rosa-sinensis flowers was made by maceration methode using etanol 70% as solvent. Fractionation of alkaloid compound was using VLC (Vaccum Liquid Chromatography) and using organic solfent mixture of methanol-ethyl acetate in variation concentration. Isolate was used as standard curve, then the concentration of H. rosa-sinensis flowers extract was determined using spectrodensitometry with silica gel as stationer phase and ethyl acetate: methanol ( 1:5 v / v) as mobile phase. The relative concentration of alkaloid was calculated by using standard curve Y = 29023.75 X + 5809.35, r = 0.996. Determination of isolate showed the relative concentration of alkaloid was 0.210 ? 0.035 % and this can be used as marker compound.

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