Protein Profile and MDA Spermatozoa Levels of Hyperglycemic Mice Fed By Bean Sprouts Extract  

Oleh:

Hidayat, Erwin ; Susanti, R. ; Marianti, Aditya

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Pharmacy vol. 26 no. 04 (Oct. 2015), page 192-199.
Topik: Protein Profiles; MDA; Hyperglicemic; Bean Sprouts
Fulltext: I03 v26 n4 p192 kelik2017.pdf (595.1KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I03.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Hyperglycemic conditions resulted in an increase in lipid peroxide would damage the structure and fluidity of membrane, causing the disturbance in membrane functions. Degradation of the membrane cause damage to important cellular components such as DNA and proteins. On the other hand, MDA (malondialdehyde) levels in diabetes mellitus patients increased compared that of control. Phytosterol and vitamin E in bean sprouts is an antioxidant that is able to maintain the integrity of cell membrane. The aim of this study is to analyze the protein profiles and MDA levels in spermatozoa of hyperglycemic mice given bean sprouts extract. This study is an experimental study with post test-only control group design. The methods of this study including the preparation of 28 mice that was divided into 4 groups: control, P1, P2, and P3 groups. The test groups (P1, P2, and P3) were induced with monohydrate alloxan at a dose of 150mg/kg for 5 days. P2 group were given bean sprouts extract at a dose of 50mg/20g and P3 group were given bean sprouts extract at a dose of 100mg/20g. Spermatozoa were collected on day 36 for MDA levels determination using TBARS and protein profiles analyzing using SDS-PAGE. The obtained data of MDA levels were analyzed using one-way ANOVA continued with LSD test, whereas the protein profiles data were qualitative descriptively analyzed. From the overall data of this study, the MDA levels showed significant differences among each groups: control P1, P2 and P3 groups were 2.43nmol/mL, 7.89nmol/mL, 5.19nmol/mL, and 3.29nmol/mL, respetively. Protein profiles analysis results showed that there was 8 protein bands in control group and P3 group; 72, 60, 49, 42, 35, 30, 28, and 23kDa; whereas that were not found any protein band in P1 and P2 groups. Bean sprouts extract contains a-tocopherol as antioxidant. Tocopherol works as free radical oxygen, lipid peroxide, and singlet oxygen. It was summarized that bean sprouts extract at a dose of 100mg/20g had influenced on recovery of protein profiles and the decrease of MDA levels in spermatozoa of hiperglycemic mice induced alloxan.

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