Phosphatidylinositol-3Kinase (PI3K) di Perbenihan Adiposit yang dipajan Glukosa Tinggi dengan Retinol  

Oleh:

Firani, Novi Khila ; Prijadi, Bambang

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 22 no. 02 (Mar. 2016), page 109-113.
Topik: Phosphatidylinositol-3Kinase; Perbenihan Adiposit; Pajanan Glukosa Tinggi; Retinol; Adipocyte Culture; High Glucose Exposure
Fulltext: I01 v22 n2 p109 kelik2017.pdf (193.84KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Retinol is one of the active forms of vitamin A. In the previous study, it was known that retinol level in serum of DM patient was lower than in healthy people, which correlated with an increase of the glucose levels in these patients. The importance of retinol in insulin signaling mechanisms that play a role in the pathogenesis of DM is still unknown. One of the components that play a role in insulin signaling on adipocytes is phosphatidylinositol-3 kinase (PI3K), which encourages the translocation of glucose transporter-4 (GLUT4) to the cell surface. The aim of this study was to know the importance of retinol therapy in the levels of PI3K enzyme on visceral adipocyte culture with high glucose exposure (25 mM) as a model of DM in vitro by determination method. Retinol therapy was given at a dose of 0.1 µM, 1 µM , and 10 µM. Measurement of PI3K level was done by ELISA method. The mean (SD) levels of PI3K enzyme were 1.91 (0.27), 0.94 (0.15), 1.98 (0.22), 1.69 (0.81), 2.04 (0.16) ng/mL respectively, for adipocyte cultures exposed to 5mM glucose (as a physiological condition), 25mM glucose, and 25mM glucose concentration with doses of retinol therapy 0.1 µM, 1 µM and10 µM. The results of this study indicated that high glucose exposure (25 mM) decreased the level of PI3K compared with adipocyte’s culture on 5 mM glucose exposure. Retinol therapy with a dose of 0.1µM, 1µM and10 µM on adipocyte culture exposed with high glucose could increase the levels of PI3K.

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