Identification of Pathogenic Leptospira in Rat and Shrew Populations Using rpoB Gene and Its Spatial Distribution in Boyolali District  

Oleh:

Widiastuti, Dyah ; Sholichah, Zumrotus ; Agustiningsih ; Wijayanti, Nastiti

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Jurnal Kesehatan Masyarakat Nasional (KESMAS) vol. 11 no. 01 (Aug. 2016), page 32-38.
Topik: Leptospira; rpoB; Spatial
Fulltext: J41 v11 n1 p32 kelik2016.pdf (499.36KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: J41.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Leptospirosis becomes health problem in Indonesia. Until April 2014, leptospirosis cases transmitted by rats were reported in Boyolali with case fatality rate (CFR) by 83.3%. Leptospira genus consists of various serovars and genetic types living in different environment. Leptospira species classification based on rpoB gene could be used as this gene has high polymorphism level. This study aimed to identify Leptospira serovars in rat population using kinship analysis based on rpoB gene polymorphism, and describe spatial distribution of rats with Leptospira positive in Boyolali District. A cross-sectional study was conducted on April 2014 at Sindon Village in Ngemplak Subdistrict and Jeron Village in Nogosari Subdistrict, Boyolali District. Polymerase Chain Reaction test was performed on 104 rat kidney samples from two locations of study. Spatial analysis was conducted to map distribution of rats with Leptospira positive. There were six positive rpoB gene samples in Rattus tanezumi, Rattus argentiventer and Suncus murinus. Five of six positive samples showed the closest genetic kinship to Leptospira borgpetersenii serovar Sejroe based on rpoB gene. One isolate did not have a close genetic kinship to any serovar included in the cluster. Spatial analysis based on home range buffer zone showed rats with Leptospira positive were found in 30 meter and 150 meter from leptospirosis patients.

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