Molecular Mechanisms of Stress-Induced Myocardial Injury in a Rat Model Simulating Posttraumatic Stress Disorder  

Oleh:

Mi Liu ; Feifei Xu ; Tianqi Tao ; Dandan Song ; Dong Li ; Yuzhen Li ; Yucheng Guo ; Xiuhua Liu

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Psychosomatic Medicine: Journal of Biobehavioral Medicine vol. 78 no. 08 (Oct. 2016), page 888-895.
Topik: Posttraumatic Stress Disorder; Myocardial Injury; Endoplasmic Reticulum Stress; Apoptosis
Fulltext: P01 v78 n8 p888 kelik2016.pdf (599.76KB)

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Perpustakaan FK Nomor Panggil: P01.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Objective: Posttraumatic stress disorder (PTSD) is an independent risk factor for cardiovascular diseases. This study investigated the molecular mechanisms underlying myocardial injury induced by simulated PTSD. Methods: Sprague-Dawley rats were randomly divided into two groups: control group (n = 18) and PTSD group (n = 30). The PTSD model was replicated using the single prolonged stress (SPS) method. On the 14th day poststress, the apoptotic cells in myocardium were assessed using both TUNEL method and transmission electron microscopy; the protein levels of the endoplasmic reticulum stress (ERS) molecules were measured by using Western blotting analysis. Results: Exposure to SPS resulted in characteristic morphologic changes of apoptosis in cardiomyocytes assessed by transmission electron microscopy. Moreover, TUNEL staining was also indicative of the elevated apoptosis rate of cardiomyocytes from the SPS rats (30.69% versus 7.26%, p < .001). Simulated PTSD also induced ERS in myocardium, demonstrated by up-regulation of protein levels of glucose-regulated protein 78 (0.64 versus 0.26, p = .017), calreticulin (p = .040), and CCAAT/enhancer-binding protein-homologous protein (0.95 versus 0.43, p = .047), phosphorylation of protein kinase RNA–like ER kinase (p = .003), and caspase 12 activation (0.30 versus 0.06, p < .001) in myocardium from the SPS rats. The ratio of Bcl-2 to Bax decreased significantly in myocardium from the SPS rats (p = .005). Conclusions: The ERS-related apoptosis mediated by the protein kinase RNA–like ER kinase/CCAAT/enhancer-binding protein-homologous protein and caspase 12 pathways may be associated with myocardial injury in a rat model simulating PTSD. This study may advance our understanding of how PTSD contributes to myocardial injury on a molecular level.

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