Effects of Growth Differentiation Factor 8 on Steroidogenesis in Human Granulosa-Lutein Cells  

Oleh:

Hsun-Ming Chang ; Lanlan Fang ; Jung-Chien Cheng ; Taylor, Elizabeth L. ; Ying-Pu Sun ; Leung, Peter C. K.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 105 no. 02 (Feb. 2016), page 520-528.
Topik: Aromatase; GDF8; Human Granulosa Cells; StAR; Steroidogenesis
Fulltext: F02 v105 n2 p520 kelik2016.pdf (794.25KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To investigate the biological role of growth differentiation factor 8 (GDF8) in the regulation of steroidogenesis in human granulosa-lutein (hGL) cells. Design: Experimental study. Setting: Academic medical center. Patient(s): In vitro fertilization patients who provided hGL cells. Intervention(s): Cultured hGL cells treated with recombinant human GDF8 for 24 hours. Main Outcome Measure(s): Expression of steroidogenic enzymes and steroid production in primary cultures of hGL cells used to investigate the effects of GDF8 via specific mRNA and protein levels examined using real time-quantitative polymerase chain reaction and Western blot analysis, respectively, and levels of estradiol and progesterone measured by enzyme immunoassays. Result(s): Extracts were prepared from cultured hGL cells after exposure to GDF8. The levels of cytochrome P450 aromatase (aromatase), the FSH receptor, and estradiol were increased, whereas steroidogenic acute regulatory protein (StAR), luteinizing hormone (LH) receptor, and progesterone levels were decreased after treatment with GDF8. In addition, follicle-stimulating hormone (FSH) stimulated the production of aromatase/estradiol, and LH induced the production of StAR/progesterone. Furthermore, pretreatment with GDF8 for 24 hours enhanced the effects of FSH on aromatase/estradiol induction, whereas GDF8 suppressed the effects of LH on StAR/progesterone stimulation. Conclusion(s): In human granulosa cells, GDF8 may play an important role in the modulation of cellular responsiveness to gonadotropins and in the regulation of ovarian steroid production, most likely as a luteinization inhibitor.

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