Sperm Deoxyribonucleic Acid Fragmentation Assessment in Normozoospermic Male Partners of Couples with Unexplained Recurrent Pregnancy Loss: A Prospective Study  

Oleh:

Bareh, Gihan M. ; Jacoby, Ethan ; Binkley, Peter ; Schenken, Robert S. ; Robinson, Randal D.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 105 no. 02 (Feb. 2016), page 329-336.
Topik: Sperm DNA; Recurrent Pregnancy Loss; Unexplained Infertility; TUNEL Assay
Fulltext: F02 v105 n2 p329 kelik2016.pdf (2.22MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL). Design: Prospective, cohort study. Setting: Academic tertiary care center. Patient(s): Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility. Intervention(s): Semen samples were collected by masturbation after 48–72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 106 spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis. Main Outcome Measure(s): Sperm DNA fragmentation in both groups. Result(s): Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7). Conclusion(s): Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

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