Separation Efficiency of a Microfluidic Sperm Sorter to Minimize Sperm DNA Damage  

Oleh:

Shirota, Kyoko ; Yotsumoto, Fusanori ; Itoh, Hiroko ; Obama, Hirotsugu ; Hidaka, Naomi ; Nakajima, Kyoko ; Miyamoto, Shingo

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 105 no. 02 (Feb. 2016), page 315-321.
Topik: Assisted Reproductive Technology; Intracytoplasmic Sperm Injection; Microfluidic Sperm Sorter; Sperm DNA Fragmentation; Sperm Chromatin Structure Assay
Fulltext: F02 v105 n2 p315 kelik2016.pdf (499.73KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To evaluate whether microfluidic sperm sorters (MFSSs) allow effective recovery of sorted motile sperm without DNA damage compared with the centrifugation and swim-up procedure. Design: Experimental laboratory study. All participants completed questionnaires regarding previous and/or current diseases, surgery, reproductive experiences, lifestyle factors, and date of the preceding ejaculation. Setting: University research laboratory. Patient(s): Male volunteers were recruited without setting conditions. Semen samples from healthy volunteers (n = 37) were collected in sterile containers by masturbation. Intervention(s): Flow cytometric measurement and sperm chromatin structure assay analysis of DNA damage after sperm preparation using MFSS and the centrifugation and swim-up procedure. Main Outcome Measure(s): Efficacy and efficiency of sperm preparation, correlation between sperm DNA fragmentation index (DFI) and semen parameters, and relationship between basic characteristics and DFI after the centrifugation and swim-up procedure. Result(s): Final sperm concentration and motility were significantly different between the centrifugation and swim-up procedure and MFSS sperm preparations. A significantly lower sperm DNA fragmentation rate was detected with MFSS compared with the centrifugation and swim-up procedure use. No correlation was observed between DFI and smoking or drinking, but significant correlations were observed between DFI and medication use and sexual abstinence duration. Conclusion(s): MFSSs can be used to efficiently and reliably prepare sperm compared with the centrifugation and swim-up procedure. Further research on the clinical use of MFSSs is required to evaluate the safety and usefulness of this device.

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