Immunochromatography Method for Rotavirus Detection Provides Good Accuracy in Diagnosis of Rotavirus Diarrhea in Children  

Oleh:

Yuniarchan, Sherly ; Darma, Andy ; Athiyyah, Alpha Fardah ; Ranuh, IGM Reza Gunadi ; Sudarmo, Subijanto Marto ; Boediono

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Folia Medica Indonesiana vol. 51 no. 2 (2015), page 142-148.
Topik: immunochromatography; rotavirus diarrhea; diagnostic accuracy; children
Fulltext: download-fullpapers-fmic6ff0e98f1full.pdf (76.67KB)

Isi artikel

Rotavirus infections are a major cause of diarrhea in children under 5 years in both the developed and developing countries. Gold standard diagnostic method for rotavirus diarrhea is RT-PCR but it is expensive and longtime. Immunochromatography is a new diagnostic method that is fast, easy and cheap but the variation in diagnostic accuracy is still very wide in various countries. The objective of this study was to evaluate the accuracy of the immunochromatography method as compared to RT-PCR method in the diagnosis of rotavirus diarrhea in children. Cross-sectional study was conducted in children aged 1-60 months with acute diarrhea hospitalized at gastroenterology ward Dr. Soetomo Hospital on January until June 2014. Patients, who had received rotavirus immunization 1 month before the test, were excluded from this study. Immunochromatography method was using BD Rota/Adeno xamine™ stick (Beckton Dickinson Japan Co., Ltd.). Identification of rotavirus by RT-PCR was performed at the Institute of Tropical Disease (ITD), Airlangga University. Sensitivity, and specificity, positive predictive value (PPV), negative predictive value (NPV) and likelihood ratio (LH) were calculated for the immunochromatography method. A total of 80 children were included.The sensitivity, specificity, PPV, NPV and LH of immunochromatography respectively were 77.6%, 63.6%, 84.9%, 51.85% and 2.13. In conclusion, immunochromatography method for rotavirus detection provides good accuracy in diagnosis of rotavirus diarrhea in children. (FMI 2015;51:142-148)

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