Molecular Analysis of Rpob Gene of Mycobacterium Tuberculosis in Patients with Suspected Pulmonary Tuberculosis in Surabaya  

Oleh:

Wiqoyah, Nurul ; Mertaniasih, Ni Made ; W, Manik Retno

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Folia Medica Indonesiana vol. 51 no. 2 (2015), page 110-113.
Topik: rpoB gene; rifampicin; Mycobacterium tuberculosis; sequencing
Fulltext: download-fullpapers-fmi9966dc6ddcfull.pdf (120.3KB)

Isi artikel

Tuberculosis caused by Mycobacterium tuberculosis is still a public health problem in Indonesia. Important initial step in an effort to control TB and MDR-TB is TB case early finding, so early and accurate diagnosis is very important to deal with TB cases quickly and accurately as well. Rapid molecular diagnostic methods based on molecular specific genes of Mycobacterium tuberculosis require the data from the genome sequence of the bacterium. RpoB gene is a gene that can be used as a bacterial diagnostic tool which is responsible for anti-TB drug rifampicin resistance that showed mutations in certain areas, and varied geography. So the sequences of the ropB gene of Mycobacterium tuberculosis need to be known. Research methods include the extraction and amplification of DNA regions rpoB gene of Mycobacterium tuberculosis clinical isolates from suspected of pulmonary TB patients, followed by sequencing. Ten isolates of Mycobacterium tuberculosis from sputum of patients suspected of pulmonary TB were obtained. Amplification of a region of the rpoB gene of Mycobactrium tuberculosis resulst in a 411 bp and sequences of Mycobacterium tuberculosis rpoB gene region showed the same sequence as the nucleotide sequence of Mycobacterium tuberculosis H37Rv. These studies concluded that the region of 411 bp rpoB gene amplification product has a 100% DNA sequence homology with Mycobacterium tuberculosis H37Rv.(FMI 2015;51:110-113)

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