Evaluation of a Human Ovarian Follicle Isolation Technique to Obtain Disease-Free Follicle Suspensions Before Safely Grafting to Cancer Patients  

Oleh:

Soares, Michelle ; Sahrari, Karima ; Amorim, Christiani Andrade ; Saussoy, Pascale ; Donnez, Jacques ; Dolmans, Marie-Madeleine

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 104 no. 03 (Sep. 2015), page 672–680.
Topik: Follicle Isolation; Leukemia; Malignant Cell Purging; Minimal Disseminated Disease; Ovarian Follicles; Ovarian Tissue Cryopreservation
Fulltext: F02 v104 n3 p672 kelik2016.pdf (1.57MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective: To evaluate the safety of our follicle isolation procedure in a model of ovarian tissue artificially contaminated with cancer cells, then to improve the procedure to effectively eliminate malignant cells from follicle suspensions without altering viability. Design: Prospective experimental study. Setting: Gynecology research unit in a university hospital. Patient(s): Ten women undergoing laparoscopy for benign gynecologic disease. Intervention(s): Follicle isolation from ovarian tissue artificially contaminated with marked fluorescent leukemic cells, either by the usual pickup technique without further treatment (group 1) or by washing three times after pickup (group 2). Main Outcome Measure(s): Evidence of leukemic cells in follicle suspensions using fluorescence microscopy and quantitative real-time polymerase chain reaction, and analysis of follicle viability. Result(s): In group 1, 196 leukemic cells were detected by fluorescence microscopy out of 499 follicles retrieved, while just one leukemic cell was found among 772 follicles after three washes. The BCR-ABL fusion transcript was detected when at least 19 cells were present in follicle suspensions; four samples were positive in group 1, and all were negative in group 2. Follicle viability was similar in both groups (95.6% vs. 96.4%). Conclusion(s): Cancer cells could inadvertently be picked up with isolated follicles in case of malignant contamination of ovarian tissue. A simple purging procedure consisting of three washes proved effective for eliminating leukemic cells while maintaining good follicle viability.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali