Human Chorionic Gonadotropin Stimulates Spheroid Attachment on Fallopian Tube Epithelial Cells Through the Mitogen-Activated Protein Kinase Pathway and Down-Regulation of Olfactomedin-1  

Oleh:

Kam-Hei So ; Kodithuwakku, Suranga P. ; Kottawatta, Kottawattage S. A. ; Chiu, Philip C. N. ; Cheung, Annie N. Y. ; Yeung, William S. B. ; Kai-Fai Lee

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 104 no. 02 (Aug. 2015), page 474–482.
Topik: Olfactomedin-1; Fallopian Tube; Tubal Ectopic Pregnancy; Wnt-Signaling; MAPK Signaling
Fulltext: F02 v104 n2 p474 kelik2016.pdf (1.7MB)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro. Design: Experimental study. Setting: Reproductive biology laboratory. Patient(s): Healthy nonpregnant women. Intervention(s): No patient interventions. Main Outcome Measure(s): Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/ß-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay. Result(s): Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/ß-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/ß-catenin signaling pathway but up-regulated Olfm1 expression. Conclusion(s): Human chorionic gonadotropin activated both ERK and Wnt/ß-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.

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