Histone Methylation and Acetylation in Ejaculated Human Sperm: Effects of Swim-Up and Smoking  

Oleh:

Seul Ki Kim ; Byung Chul Jee ; Seok Hyun Kim

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 103 no. 06 (Jun. 2015), page 1425–1431.
Topik: Spermatozoa; Epigenomics; Histone; Methylation; Smoking
Fulltext: F02 v103 n6 p1425 kelik2016.pdf (320.26KB)

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Perpustakaan FK Nomor Panggil: F02.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To assess global methylation and acetylation level before and after swim-up in ejaculated human sperm. Design: Prospective experimental study. Setting: University-based hospital. Patient(s): Semen samples were obtained from 50 healthy volunteers (single sample per person) between December 2012 and May 2014. Intervention(s): The semen samples were processed by the swim-up method. Main Outcome Measure(s): Sperm motility, strict morphology, DNA fragmentation index (DFI) by TUNEL, abnormal chromatin structure by toluidine blue staining, immunostaining of 8-hydroxy-20-deoxyguanosine (8-OHdG) as an oxidative stress marker, immunostaining of 5-methyl-cytosine (5-mC) as a measurement of methylation level and immunostaining of six acetylation markers (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) before and after swim-up. Result(s): After swim-up, motility and the percentage of normal form was significantly elevated. In the swim-up fraction, DFI and the percentage of abnormal chromatin structure were significantly lower and 8-OHdG levels were significantly higher. The 5-mC levels were significantly lower in the swim-up fraction, whereas no changes were observed in the positivity of six acetylation markers. Elevated percentage of normal form and decreased DFI and 5-mC levels were observed only in nonsmokers; 8-OHdG level was significantly higher in the swim-up fraction only from smokers. After swim-up, 5-mC levels were positively correlated with DFI. Conclusion(s): This is the first study demonstrating decreased methylation and unchanged acetylation patterns in human sperm after swim-up preparation. (Fertil Steril_ 2015;103: 1425–31. _2015 by American Society for Reproductive Medicine.)

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