Induced-Coagulated Plasma-Fibrin Gels as a Biological Scaffold for Cell Attachment and Proliferation of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC)  

Oleh:

HERMANTARA, RIO ; Kerans, Fiano A. ; Rizal ; Herningtyas, E. Henny ; Lazuardi, Lutfan

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 19 no. 02 (2014), page 159-167.
Topik: Fibrin gels; mesenchymal stem cells; activated partial thromboplastin time; cell attachment and proliferation
Fulltext: 9310-17276_her.pdf (369.99KB)

Isi artikel

Fibrin gels are an ideal natural biological scaffold for tissue engineering because they are biocompatible, biodegradable, and have many biological surface markers. However, most research on ? brin gels used commercial ? brin kits that could be costly and limited in some areas. In this study, ? brin gels were made by inducing blood coagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partial thromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-? brin gels was evaluated for its ability to enhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachment and proliferation. Fibrinogen concentration had in? uence on cell attachment, where only 50% of the cells could attach to 77 mg/dl ? brinogen gels whereas 93% cells adhered to 154 mg/dl ? brin gels. There were no signi? cant differences in cell proliferation on polysterene culture dish and ? brin gels (p>0.05). These results showed that iCoplas-? brin gels could be used as a ? brin-based scaffold, yielding no signi? cant difference than polysterene-tissue culture dish cultures in cell attachment and cell proliferation on 154 mg/dl ? brinogen concentration.

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