The Assessment of Genetic Variability and Taxonomic Af? Nity of Local Pummelo Accessions from Yogyakarta, Indonesia Based on RAPD  

Oleh:

Susandarini, Ratna ; Arifati, Rina ; Chasani, Abdul Razaq ; Subandiyah, Siti

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 19 no. 02 (2014), page 153-158.
Topik: Citrus maxima; taxonomic af? nity; genetic variability; RAPD.
Fulltext: 9309-17272_her.pdf (578.45KB)

Isi artikel

Pummelo (Citrus maxima) is one of three biotypes considered as true species within the genus Citrus. A major issue of pummelo taxonomy in Indonesia is the high number of cultivars showing variability in phenotypic characters but of unknown genetic diversity. In this study, the assessment of genetic variability and taxonomic af? nity of local accessions of C. maxima from Yogyakarta was examined based on RAPD ? ngerprinting. The availability of universal primers and technical simplicity makes RAPD as a molecular tool of choice for the assessment of genetic variability at various taxonomic levels. In this study, 13 accessions of C. maxima collected from Yogyakarta were observed for their genetic variability. An additional three registered cultivars were included for comparative purpose. Two decamer primers used for the ampli? cation of DNA produced 222 bands with 174 of them were polymorphic. The data was subjected to cluster analysis to observe the grouping of accessions and taxonomic af? nity. Results indicated high genetic variability among accessions. The dendrogram constructed using UPGMA method based on simple matching coef? cient showed two main clusters were which was in line to morphological characters. The grouping of accessions showed clear differentiation between accessions bearing white and those with reddish fruit ? esh, and thus demonstrates taxonomic value of this study in recognizing important agronomic character for this tropical fruit crop.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali