In Vitro Formation of 8-hydroxy-2 ' -deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2 ' -deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone  

Oleh:

Budiawan ; Widiastuti, Dwi Retno

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Sains vol. 19 no. 4 (Dec. 2015), page 167-176.
Topik: 2; 6-di-tert-butil-p-benzoquinone; DNA adduct; 8-hydroxy-2'-deoxyguanosine; propyl gallate
Fulltext: 5171-9774_her.pdf (690.36KB)

Isi artikel

Oxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2'-deoxyguanosine (dG). PG in the presence of CuCl increased the 8OHdG formation in calf thymus DNA by around 9.17 times as compared to the control (untreated DNA). In the presence of CuCl 2 at 1.28×10 -5 M, the 8-OHdG per dG ratio resulting from the reaction of dG with PG at various concentrations (20–150 ppm) ranged from 75.50 to 312.06 8-OHdG per 10 167 5 dG. The 8-OHdG formation increased when the PG concentration was increased from 20 ppm to 80 ppm, and then, it began to plateau around 80 ppm. On the other hand, BHT-quinone increased the formation of 8-OHdG in the presence of CuCl by 0.05 times as compared to the control (untreated DNA). LC-MS/MS analysis was used to identify the molecular structure of 8-OHdG, which had a base peak (M +. + 1) at m/z = 284 and two main fragments at m/z = 167.9 and m/z = 139.9.

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