Effect of purified gambir leaves extract to prevent atherosclerosis in rats  

Oleh:

Aini, Nurul ; Yunarto, Nanang

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Health Science Journal of Indonesia vol. 06 no. 02 (Dec. 2015), page 105-110.
Topik: gambir; catechin; antiatherosclerosis

Ketersediaan

Perpustakaan PKPM Nomor Panggil: H25 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: H25.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Background: Atherosclerosis is a risk factor for coronary heart disease (CHD). Cetechin have high antioxidant activity that can prevent atheroclerosis. Gambir (Uncaria gambir, Roxb.) leaves extract have high cathecin content therapy potentially inhibiting atherosclerosis. This research was aimed to examine effect of purified gambir leaves extract to prevent atherosclerosis in rats. Methods: The experimental laboratory study was conduxted in Pharmacy Laboratory and Animal Laboratory. National Institute of Health Research and Development, Ministry of Health, Republic Of Indonesia in 2014.Gambir leaves extract were purified to gain optimum catechin. Afterwards, antioxidant activity was tested usng 2.2-diphenyl-1picrylhydrazyl (DPPH) method. With ascorbic acid as positive control. Thirty six white male Sprague Dawley rats aged 2.5 months were randomly divided into six groups, i.e. normal control group, negativecontrol group (aquadest), positive control group (atorvastatin 2mg/200 g bw), extract dose I (20mg/200 g bw), dose II (40mg/200 g bw), dose III (80mg/200 g bw). The rats were given high fat diet and treatment according to their group for 60 days, except for normal control group. Results: Cathecin content in the purified gambir leaves extract was 92,69%. From antioxidant activity test, IC50 was found to be 11,76 µg/Ml. Anti-antherosclerotic activity study shown that compared to negative control, all three doses of purified gambir leaves extract were able to prevent atherosceloris through inhibition of aortic wall thickening and foam cell formation due to high fat diet (p<0.05). Anti-arherosclerotic activity increased with increasing dose. Conclusion: Gambir leaves purified extract had the effect of preventing the thickening of the walls and foam cell information rat aorta.

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