Optimization of Culture Conditions for Production of ß-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation  

Oleh:

Ratnakomala, Shanti ; Yopi ; Suhartono, Maggy T. ; Meryandini, Anja ; Prasetya, Bambang

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI - non-atma jaya
Dalam koleksi: Annales Bogorienses vol. 18 no. 02 (Dec. 2014), page 1-11.
Topik: ß-mannanase; actinomycetes; Nonomuraea sp. ID06-379; optimization medium; submerged substrate fermentation
Fulltext: AA770118022014.pdf (668.98KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: AA77 Non-tandon: tidak ada Tandon: 1

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Isi artikel

The objective of this study was to investigate the effect of media compositions on the production of ßmannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on ß-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing ß-mannanase (1.075 ± 0.006 U/ml), (NH 4 ) 2 HPO as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH 4 4 ) 2 HPO were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of ß-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH 4 , under submerged fermentation with shaking at 120 rpm and 28?C for 2 days incubation.

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