Inter- And Intraspecifi C Variation Of Chloroplast Mini- and Microsatellites DNA in the Four Closed Related Acacia Species  

Oleh:

Widyatmoko, AYPBC ; Shiraishi, Susumu

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Biotechnology vol. 19 no. 01 (2014), page 1-11.
Topik: Acacia; microsatellite; variation; chloroplast DNA
Fulltext: 264-703-1-PB_Ros.pdf (409.71KB)

Isi artikel

Mini- and microsatellites of four Acacia species, A. aulacocarpa, A. auriculiformis, A. crassicarpa and A. mangium were investigated on four non-coding regions of cpDNA, the intron of trnL, and the intergenic spacers of trnL - trnP, trnD - trnY, and trnP – trnW. Nine single base substitutions and six informative miniand microsatellites were detected in the the four cpDNA non-coding regions. Based on the substitutions and mini- and microsatellites, ten cpDNA haplotypes (A - J) could be distinguished. Acacia auriculiformis possessed fi ve haplotypes, A. aulacocarpa, four haplotypes, and A. crassicarpa, three haplotypes. All samples of A. mangium possessed the same haplotype. Mini- and microsatellites recognized in this study can be used for species identifi cation of the four Acacia species. The ten haplotypes could divided the four species into 2 groups, A. aulacocarpa-A.crassicarpa group and A. auriculiformis-A. mangium group. By developing the PCR-based markers based on the sequence information, many experiments can be carried out for the Acacia improvement programs.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali